Table 1 Primer sequences for methylation analysis

From: Tactile stimulation reverses painful stimuli outcomes via PVN-VTA oxytocin circuitry and dopaminergic regulation

Gene name

Primer position

Sequences (5’-3’)

Annealing temperature

Product (bp)

CpGs in product

Drd1

1368-F

TTAGGAGGGGAATTATTTGTTAGGT

69.1

228

7

1595-R

AAATCCCTAAAACCCCATATATCAC

Drd2

1867-F

ATTTTGTTAGTGTTTGGGTTTTTTT

63.5

185

6

2051-R

AATTCTACCTCTCCAAATCATCATC

Nr3c1

1496-F

ATAAAATTTTAAGGGGAGGAGTTAT

68.7

207

4

1702-R

TTCCCATCACTTTTATTTCTATCTC

Oxtr

1090-F

GGAGATAGGATGGTTAGTATTAGTG

66.3

168

9

1257-R

TAACCCAACAACCTCAAAACTCTAC

  1. “F” stands for upstream primer; “R” stands for downstream primer.