Fig. 1: Identification of suitable sites for incorporating pAcF. | Communications Biology

Fig. 1: Identification of suitable sites for incorporating pAcF.

From: Noncanonical amino acid-aided synthesis of anti-PD-L1 bispecific nanobody for colon cancer immunotherapy

Fig. 1: Identification of suitable sites for incorporating pAcF.The alt text for this image may have been generated using AI.

A General scheme for generating BsNbs; B Flowchart for computationally selecting suitable ncAA incorporation sites; C Estimated Cartesian_ddG and Flex_ddG values for PV2 and PV3 using Rosetta Cartesian_ddG and Flex_ddG. Pink dots, amino acids predicted to be suitable pAcF incorporation sites; Orange dots, amino acids predicted to significantly influence stability. Amino acids in CDR region are removed; D, Structures of PV2 and PV3 showing the positions of pAcF incorporation.

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