Fig. 4: Quantification of phospholipids in ∆3, ∆3∆cfa, and ∆3∆fabR.

a Phospholipids were extracted from mutants of E. coli, and TLC analysis was performed. b Phosphorus assay was performed for each of the following: CL, PE, PG, and PS. ∆3/nhaA (white bar), ∆3∆cfa/nhaA (grey bar), ∆3∆fabR/nhaA (black bar). Data represent mean ± SE (n = 3). Statistical analyses was conducted using two-sample t-tests (P ≥ 0.05). n.s. indicates non-significance.