Table 1 Comparison of single-cell RNA m6A methylation detection methods
From: Systematic evaluation of tools used for single-cell m6A identification
| Â | sn-m6A-CT | scm6A-seq | scDART-seq | Scm6A |
|---|---|---|---|---|
Object | Native population of cell nucleus | Mouse embryonic cells at cleavage stage | HEK293T stable cell lines | 25 human cell lines |
Simultaneous profiling of m6A methylomes and transcriptomes | Yes | Yes | Yes | No |
Flux | High throughput | Low throughput | High throughput | High throughput |
Prediction principle | Antibody based | MeRIP and RNA multiplex labeling technique | SMART-seq2 | Machine learning algorithm |
m6A Positioning | Yes | Yes | Yes | Yes |
m6A Quantitative | No | Yes | Yes | Yes |
Expenditure | High | High | High | low |
Time-consuming | Long | Long | Long | Fast |
