Fig. 4: Localization of topological defects and differentiation pattern in stretched aggregates.
From: Shaping 3D minimal model tissues with mechanical constraints to orchestrate muscle differentiation
a 3D Reconstruction from confocal images of stretched aggregates. Actin filaments are made visible using SiR-actin labelling. Topological defects are highlighted in blue (half integer defects) and in red (integer defect). Scale bar = 100 μm. b Representative confocal image of troponin T expression (magenta) superimposed with contour integration of actin filaments labelled with phalloidin (grey). The section corresponds to the attached part of the aggregate. Scale bar = 100 μm.
