Fig. 6: Validation of interactions between three nanobodies and SOD1 or SOD1-nanobody complexes using isothermal titration calorimetry (ITC). | Communications Biology

Fig. 6: Validation of interactions between three nanobodies and SOD1 or SOD1-nanobody complexes using isothermal titration calorimetry (ITC).

From: Structural mechanisms and insights on multiple nanobodies binding diverse SOD1 epitopes

Fig. 6

A–D Binding of Nb1 to SOD1, SOD1-Nb2, SOD1-Nb3, and SOD1-Nb2-Nb3; E–H Binding of Nb2 to SOD1, SOD1-Nb1, SOD1-Nb3, and SOD1-Nb1-Nb3; I–L Binding of Nb3 to SOD1, SOD1-Nb1, SOD1-Nb2, and SOD1-Nb1-Nb2. Panels show ITC profiles with binding isotherms and corresponding thermodynamic parameters.

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