Table 2 The binding parameters and relative thermodynamic parameters between nanobodies and SOD1 or SOD1-nanobody complexes by isothermal titration calorimetry
From: Structural mechanisms and insights on multiple nanobodies binding diverse SOD1 epitopes
N (Site) | KD (M) | ΔH (kcal/mol) | −TΔS (kal/mol) | ΔG (kcal/mol) | |
|---|---|---|---|---|---|
Nb1 to SOD1 | 0.950 ± 6.7 × 10−2 | 529 × 10−9 ± 65.3 × 10−9 | −14.2 ± 0.184 | 5.60 | −8.56 |
Nb2 to SOD1 | 0.978 ± 1.9 × 10−3 | 23.2 × 10−9 ± 3.80 × 10−9 | −16.3 ± 0.068 | 5.85 | −9.43 |
Nb3 to SOD1 | 1.38 ± 3.4 × 10−3 | 48.9 × 10−9 ± 11.4 × 10−9 | −4.82 ± 0.032 | −5.16 | −9.98 |
Nb1 to SOD1-Nb2 | 1.10 ± 2.7 × 10−2 | 670 × 10−9 ± 183 × 10−9 | −10.5 ± 0.436 | −2.09 | −8.42 |
Nb1 to SOD1-Nb3 | 1.43 ± 2.9 × 10−2 | 1.39 × 10−6 ± 270 × 10−9 | −15.8 ± 0.584 | 7.85 | −8.00 |
Nb2 to SOD1-Nb1 | 0.965 ± 6.5 × 10−3 | 17.7 × 10−9 ± 5.35 × 10−9 | −13.6 ± 0.283 | 2.98 | −10.6 |
Nb2 to SOD1-Nb3 | 0.974 ± 9.0 × 10−3 | 120 × 10−9 ± 27.4 × 10−9 | −13.2 ± 0.228 | 3.77 | −9.44 |
Nb3 to SOD1-Nb1 | 1.41 ± 2.1 × 10−2 | 282 × 10−9 ± 77.7 × 10−9 | −7.37 ± 0.208 | −1.56 | −8.94 |
Nb3 to SOD1-Nb2 | 0.924 ± 7.8 × 10−3 | 5.38 × 10−9 ± 16.0 × 10−9 | −4.93 ± 0.115 | −6.36 | −11.3 |
Nb1 to SOD1-Nb2-Nb3 | 1.00 ± 7.7 × 10−3 | 127 × 10−9 ± 22.6 × 10−9 | −10.3 ± 0.144 | 0.884 | −9.41 |
Nb2 to SOD1-Nb1-Nb3 | 1.06 ± 5.5 × 10−3 | 14.7 × 10−9 ± 4.01 × 10−9 | −11.6 ± 0.121 | 0.957 | −10.7 |
Nb3 to SOD1-Nb1-Nb2 | 1.01 ± 1.1 × 10−2 | 29.5 × 10−9 ± 12.5 × 10−9 | −6.83 ± 0.139 | −3.44 | −10.3 |
