Fig. 1: 600 lux blue light exposure impaired spatial memory retrieval.
From: Neural mechanisms of the V2M–PrL pathway mediate blue light-induced spatial memory impairment
A Schematic diagram of the procedure for exposing mice to different wavelengths of light for 21 days followed by behavioral testing. B Illustration of light exposure in mice. C Schematic of the Y-maze behavioral test in mice. D Heatmap of Y-maze behavioral test in mice. E Statistical graph of Y-maze behavioral test in mice. Control group received 150 lux white light; WLED, GLED, and BLED groups received 600 lux white, green, and blue light, respectively (n = 6 mice per group). F Statistical graph of Y-maze behavioral test in mice exposed to different light for 21 days (n = 6 mice per group). Data analyzed by one-way ANOVA revealed a significant main effect of light exposure on the time spent in the novel arm of the Y-maze (F (3,20) = 7.170, P = 0.0019). Post-hoc Tukey’s test further indicated that the time spent in the novel arm was significantly lower in the BLED group compared to the Control group (P = 0.0037), the GLED group (P = 0.0039), and the WLED group (P = 0.0235). No significant differences were observed in the other pairwise comparisons. G Schematic of the novel object location test (NOL) in mice. Heatmap (H) and Statistical graph (I) of novel object location test results in mice (n = 6 mice per group). During the Encoding phase, an unpaired t-test detected no significant difference between the Control and BLED groups (t (10) = 0.4437, P = 0.6667). During the Recall phase, an unpaired t-test detected a significant difference between the Control and BLED groups (t (10) = 2.278, P = 0.046). J Schematic of the elevated plus-maze behavioral test (EPM) in mice. K, L Heatmap and statistical graph of EPM in mice (n = 6 mice per group). M Schematic of the open field test (OFT) in mice. N, O Heatmap and statistical graph of OFT in mice (n = 6 mice per group). All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; n.s. not significant. The actual time exploring object A or B during encoding and recall session are presented in Supplementary Information (Table 1). Details of the statistical analyses are presented in Supplementary Information (Table 2).
