Fig. 5: Dissection of the V2MGlu → PrLGABA→Glu neural pathway.
From: Neural mechanisms of the V2M–PrL pathway mediate blue light-induced spatial memory impairment
A Schematic diagram of in vivo calcium signal fiber photometry recording of PrLGlu. B Representative images of in vivo optogenetic activation of V2MGlu neurons while recording PrLGlu signals (left: V2M fiber implantation site; right: PrL fiber implantation site; scale bar: 200 µm). C Heatmaps showing trials aligned to the onset of photostimulation (473 nm, 100 ms, white dashed line). D Representative traces of GCaMP6m-expressing signals in PrLGlu neurons of C57 mice. The thick green and blue lines indicate the mean, and the light shading represents the standard error of the mean (SEM). E Schematic diagram of in vivo calcium signal fiber photometry recording of PrLGABA. F Representative images of in vivo optogenetic activation of V2MGlu neurons while recording PrLGABA signals (left: V2M fiber implantation site; right: PrL fiber implantation site; scale bar: 200 µm). G Heatmaps showing trials aligned to the onset of photostimulation (473 nm, 100 ms, white dashed line). H Representative traces of GCaMP6m-expressing signals in PrLGABA neurons of Vgat-Cre mice. The thick green and blue lines indicate the mean, and the light shading represents the standard error of the mean (SEM). I Schematic diagram of in vivo calcium signal fiber photometry recording of PrLGlu. J Representative images of in vivo optogenetic activation of V2MGlu neurons while recording GCaMP6m-expressing PrLGlu signals (left: V2M fiber implantation site; right: PrL fiber implantation site; scale bar: 200 µm). K Heatmaps showing trials aligned to the onset of photostimulation (473 nm, 100 ms, white dashed line). L Representative traces of GCaMP6m-expressing signals in PrLGlu neurons of Vgat-Cre mice. The thick red and blue lines indicate the mean, and the light shading represents the standard error of the mean (SEM). M Schematic of viral injection and the recording configuration. N Representative images showing that terminals of ChR2 (red) from the V2M were frequently detected in GABAergic neurons (green) in the PrL. Representative traces (O) and summarized data (P) of light-evoked currents recorded from the PrL before and after CNQX (10 μM). All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; n.s. not significant. Details of the statistical analyses are presented in Supplementary information (Table 2).
