Fig. 6: Deficiency of dmrt1 in gonadal somatic cells drove exogenous tGSCs to differentiate into oocytes. | Communications Biology

Fig. 6: Deficiency of dmrt1 in gonadal somatic cells drove exogenous tGSCs to differentiate into oocytes.

From: Cyprinid Juji (Gobiocypris rarus) as a model fish to study germ cell development and gonadal differentiation

Fig. 6

A Schematic of tGSCs transplantation. All dmrt1-/- host gonads developed ovaries. B dnd MO injected controls. Scale bar: 300 µm. C–E Fluorescence imaging of EGFP-positive hosts at 6 dpf, 10 dpf, and 30 dpf, respectively. tGSCT: Testicular germline stem cell transplantation. Scale bars: 300 µm and 750 µm, respectively. F, I, and L Fluorescence imaging of host gonads at 160 dpf. Scale bars: 3 mm. G, J, and M Histological analysis of host gonads at 160 dpf. Scale bars: 200 µm, 50 µm, and 200 µm, respectively. H, K, and N Offspring of hosts at 10 dpf. At this stage, the fluorescence observed in female offspring still includes maternal contribution, while the fluorescence in male offspring is solely derived from zygotic expression. Scale bars: 500 µm. O Sex ratio of dmrt1+/- and dmrt1-/- hosts after maturation (n = 15 dmrt1+/-, n = 12 dmrt1-/-). P Fertility analysis of female adult hosts of different genotypes crossed with WT males (n = 3 for each group, unpaired two-tailed Student′s t-test exhibited no significant difference, mean ± SEM).

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