Fig. 6: The vSubglu-ATN circuit predominates the anti-epileptogenesis effect.
From: Ventral subicular pyramidal neurons contribute to epileptogenesis via anterior thalamic circuit
a1–c1 Diagram of optogenetic inhibition in BLA, LS, and ATN, left; viral expression and optic fibers in BLA, LS, and ATN, respectively. a2–c2 Effect of optogenetic inhibition of vSubglu-BLA, LS, and ATN on the development of seizure stage, Two-way ANOVA, vSub-BLA: n = 8 mice in Light-OFF and Light-ON, Light ON vs. Light OFF, F(1,14) = 0.04214, P = 0.8403, ns; vSub-LS: n = 7 mice in Light-OFF and Light-ON, Light ON vs. Light OFF, F(1,12) = 0.04998, P = 0.4931, ns; vSub-ATN: n = 7 mice in Light-OFF and Light-ON, F(1,12) = 24.48, ***P = 0.0003. a3–c3 Effect of optogenetic inhibition of vSubglu-BLA, LS, and ATN on the development of ADDs, Two-way ANOVA, vSub-BLA: Light ON vs. Light OFF, F(1,14) = 1.036, P = 0.3260, ns; vSub-LS: Light ON vs. Light OFF, F(1,12) = 0.05663, P = 0.8159, ns; vSub-ATN: F(1,12) = 11.85, **P = 0.0049. a4–c4 Numbers of stimulations needed to fully-kindled, Unpaired t-test, vSub-BLA: F(1,14) = 1.029, P = 0.1236, ns; vSub-LS: Light ON vs. Light OFF, F(1,12) = 1.838, P = 0.5232, ns; vSub-ATN: F(1,12) = 2.773, *P = 0.0146. a5–c5 Comparison of numbers of stimulations staying in FSs and GSs, One-way ANOVA with post hoc Dunnett’s test, vSub-BLA FS: F(1,14) = 2.446, P = 0.7768, ns, GS: F(1,14) = 2.446, P = 0.2906, ns; vSub-LS FS: F(1,12) = 1.866, P = 0.3558, ns, GS: F(1,12) = 2.521, P = 0.9628, ns; vSub-ATN FS: F(1,12) = 6.061, *P = 0.0070, GS: F(1,12) = 5.838, P = 0.3298, ns.
