Fig. 3: Inhibition of tumor EV secretion reduces the accumulation of vitamin A metabolites in the liver.

A Schematic of the animal model. B Western blot analysis of Rab27a expression in MDA-MB-231 and MDA-MB-231/shRab27a #1 and #2 cells. C Nano-flow cytometer analysis of EV number from MDA-MB-231 and MDA-MB-231/shRab27a #2 cells. D SRS images of liver tissues from TB and Rab27a KD TB mice in the C–H stretching region. E Quantification of LD size in lipid-rich regions. F Quantification of lipid intensity at 2855 cm⁻¹ for each LD. G Intensity ratio of 3015 cm⁻¹ and 2855 cm⁻¹ peaks, representing relative unsaturated lipid content in each LD. H Mean SRS spectra of lipid-rich and protein-rich regions in the fingerprint region. I SRS images of liver tissues from TB and Rab27a KD TB mice at 1595 cm⁻¹. J Quantification of vitamin A levels in each LD within liver tissues from TB and Rab27a KD TB mice, measured by the ratio of SRS intensity at 1595 cm⁻¹ to 1455 cm⁻¹. Error bars represent SD. ****p < 0.0001. Scale bars: 20 μm. Part of the figure is created in BioRender. Lee, H. (2025) https://BioRender.com/jozm8oh.