Fig. 2: Positive and negative selection of mutant genes in the dorsal epidermis.
From: The dynamics of mutational selection in cutaneous squamous carcinogenesis
a, b Nonsynonymous: synonymous (dN/dS) ratios in dorsal skin. Mutations in all mice across all time points are pooled; 1 is neutral. a Genes under positive selection (q < 0.01 and dNdS ratio >1.3 for nonsense/splice mutations). b Negatively selected genes (q < 0.01 and dN/dS ratio <0.6 for nonsense/ splice mutations). Source Data: Supplementary Data 6. c–e Nonsynonymous (black points) and synonymous (gray points) VAF distribution for Trp53 (c), Notch1 (d), and Fat1 (e), p values determined by two-tailed Wilcoxon test. Red lines indicate the median. Source Data: Supplementary Data 7. f–h Percentage area of dorsal epidermis colonized by cells mutant for Trp53 (f), Notch1 (g), and Fat1 (h), estimated from summed variant allele frequency at each time point. Data shows the average across all mice for each time point, n = 4 mice for each time point. Upper and lower bounds reflect uncertainty in copy number and multiple mutations per cell. Source Data: Supplementary Data 8.
