Fig. 4: HOXA2 overexpression inhibits oxidative stress, ECM accumulation and EMT via suppressing ER stress in TGF-β1-induced HK-2 cells. | Communications Biology

Fig. 4: HOXA2 overexpression inhibits oxidative stress, ECM accumulation and EMT via suppressing ER stress in TGF-β1-induced HK-2 cells.

From: HOXA2 exerts anti-renal fibrosis effects through reducing endoplasmic reticulum stress via the upregulation of SIRT1

Fig. 4

A The proteins expression levels of CHOP, GRP78, E-cadherin, α-SMA, FN and COL I were assessed using Western blot analysis after forty-eight hours treatment with HOXA2 overexpression plasmids in TGF-β1-induced HK-2 cells. B Cell proliferation was detected with a CCK-8 assay after transfection with the HOXA2 overexpression plasmidsat 0, 24, 48 and 72 h. C Representative images of HOXA2 overexpression improved ER and mitochondrial injury in TGF-β1-induced HK-2 cells were observed by TEM (×20.0k). Bar = 500 nm. D H2-DCFDA staining showed that HOXA2 overexpression plasmids alleviated TGF-β1-induced ROS generation in HK-2 cells (×200). Bar = 100 μm. E The proteins expression levels of CHOP, GRP78, E-cadherin, α-SMA, FN and COL I were assessed using Western blot analysis after forty-eight hours treatment with HOXA2 overexpression plasmids in thapsigargin-induced HK-2 cells. In all panels, the data are representative of three independent experiments (n = 3). Data are presented as the mean ± SD. **P < 0.01, NS not significant.

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