Fig. 7: Effect of HOXA2 on the expression of SIRT1 in UUO mice kidney. | Communications Biology

Fig. 7: Effect of HOXA2 on the expression of SIRT1 in UUO mice kidney.

From: HOXA2 exerts anti-renal fibrosis effects through reducing endoplasmic reticulum stress via the upregulation of SIRT1

Fig. 7

A qRT-PCR analysis detected the mRNA expressions of HOXA2 and SIRT1 in the mice renal tissues of each group. B Western blot analysis detected the protein expression of HOXA2 and SIRT1 in the mice renal tissues of each group. C IHC detection of HOXA2 and SIRT1 in the mice renal tissues of each group and average optical density of each index (×200). Bar = 100 μm. D Dual immunofluorescent staining of HOXA2 and segment-specific tubular markers in the mice renal tissues from sham group. LTL, lotus tetragonolobus lectin; PNA, peanut agglutinin; DBA, dolichos biflorus agglutinin. Arrows indicate positive tubules with colocalization of HOXA2 and specific tubular markers (×200). Bar = 100 μm. Data are presented as the mean ± SD of each mouse (n = 6 mice in each group). **P < 0.01, NS not significant.

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