Fig. 1: Mitochondrial Fe-S cluster biosynthesis.
From: Nanobodies as tools for studying human frataxin biology
A, B Two views of the cryoEM structure of the Cys desulfurase supercomplex (PDB ID: 6NZU) are presented. Cys desulfurase NFS1, ACP, ISD11, ISCU2, and FXN are shown in a surface representation. Only part of the supercomplex is shown in (C), the Cys381 of the NFS1 enzyme, ISCU2, and FXN are shown—additionally, the PLP cofactor, FXN Trp155, and ISCU2 assembly site. The distance between PLP and Cys381 is ∼15 Å, and between Cys381 and the ISCU2 assembly site it is ∼15 Å. ISD11 and ACP subunits were omitted for clarity. D A nanobody (VHH domain) structure scheme shows the three complementarity-determining regions: CDR1, CDR2, and CDR3. The N- and C-termini are indicated.
