Table 1 X-ray crystallographic data collection and refinement statistics of FXN-nanobodies complexes
From: Nanobodies as tools for studying human frataxin biology
FXN:16C10 | FXN:6B1 | FXN:4A7 | |
|---|---|---|---|
Data collection | |||
Space group | P212121 | P63 2 2 | P212121 |
Cell dimensions | |||
a, b, c (Å) | 57.90, 81.10, 107.55 | 97.25, 97.25, 128.11 | 38.52, 48.15, 111.43 |
α, β, γ (°) | 90, 90, 90 | 90, 90, 120 | 90, 90, 90 |
Wavelength | 0.97926 | 0.97926 | 0.97926 |
Resolution (Å) | 64.75–1.99 (2.18–1.99)a | 84.22–1.48 (1.58–1.48)a | 55.71–1.25 (1.33–1.25)a |
Rsym or Rmerge | 0.164 | 0.058 | 0.046 |
CC(1/2) | 0.99 (0.39) | 0.99 (0.57) | 0.99 (0.60) |
Mean I/σ (I) | 14.2 (1.4) | 33.5 (1.5) | 23.6 (1.3) |
Completeness (%) | 91.7 (53.2) | 96.8 (74.5) | 89.3 (43.0) |
Redundancy | 11.5 (5.4) | 38.4 (41.1) | 10.7 (3.8) |
Refinement | |||
Resolution (Å) | 64.75–2.00 | 50.98–1.48 | 55.71–1.50 |
No. reflections | 25977 | 50812 | 34017 |
Rwork/Rfree | 0.17/0.22 | 0.21/0.22 | 0.18/0.20 |
Molecules per a.u. | 2 | 1 | 1 |
No. atoms | |||
Protein | 1888 | 1935 | 1852 |
Ligands | 0 | 0 | 0 |
Water | 902 | 161 | 196 |
B-factors | 28.8 | 25.6 | 18.2 |
Ramachandran | |||
Favored/allowed (%) | 96.67/2.71 | 98.00/2.00 | 98.71/0.86 |
Disallowed (%) | 0.62 | 0.41 | 0.43 |
R.m.s. deviations | |||
Bond lengths (Å) | 0.007 | 0.006 | 0.006 |
Bond angles (°) | 0.996 | 0.799 | 0.845 |
PDB code | 9HO6 | 9HO4 | 9HO5 |
