Fig. 2: Identification of immature low-density neutrophils using CD10, Ig κ, and CD49d. | Communications Biology

Fig. 2: Identification of immature low-density neutrophils using CD10, Ig κ, and CD49d.

From: Multimodal single-cell protein and RNA profiling unveils dysregulated immature neutrophil dynamics in gestational diabetes mellitus

Fig. 2: Identification of immature low-density neutrophils using CD10, Ig κ, and CD49d.

A Experimental workflow schematic illustrating the labeling and flow cytometry analysis of LDNs and HDNs using the indicated discriminating markers. B Representative histograms (upper) and statistical analysis (lower) of flow cytometric data for CD10, Ig κ, CD49d, and CD63 expression on LDNs and HDNs in pregnancies at 24–28 weeks of gestation (n = 27). Gray dashed lines indicate unstained (Neg) controls. C Flow cytometric gating strategy for demultiplexing HDNs and LDNs (left), with representative plots of CD10, CD49d, and Ig κ expression on LDNs and HDNs (middle); bar graphs (right) showing percentages of cells with different marker combinations in each subset. D Representative ROC curves for marker combinations in distinguishing LDNs from HDNs. E ROC curve analysis evaluating three-marker combinations (CD10, Ig κ, and CD49d) in distinguishing LDNs from HDNs in pregnancies (n = 27); the area under the curve (AUC) was calculated (95% CI: 0.985–0.992). F Proportion of LDNs in the CD10−CD49d+Ig κ+ gate and HDNs in the CD10+CD49d−Ig κ− gate. Error bars: means with SEM. Statistics: unpaired Student’s t test (B). ***P < 0.001.

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