Fig. 1: TUBA4A and TUBB4B promote the abilities of migration and proliferation, and a tendency of contractile-to-synthetic phenotypic switching in MMD.
From: Organoid Modeling and Single-Cell Profiling Reveal Smooth Muscle Cell Migration in Moyamoya Disease
A ELISA validation of differentially expressed proteins identified in serum proteomics. B The bar chart showing the expression levels of TUBA4A in the serum validation cohort (mean ± SD, n = 15 biologically independent samples). C The bar chart showing the expression levels of TUBB4B in the serum validation cohort (mean ± SD, n = 15 biologically independent samples). D Schematic diagram of the cell model construction for TUBA4A/TUBB4B overexpression and GJA1 knockdown in HBVSMCs, and the co-culture of smooth muscle cell (SMC) and endothelial cell (EC). E 5-Ethynyl-2′-deoxyuridine (EDU) staining of the TUBA4A/TUBB4B overexpression HBVSMC. Colors: EDU (red), 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale = 20 µm. The bar chart of H shows the proportion of proliferation HBVSMC (mean ± SD, n = 3 biologically independent samples). F Scratch assay showing the migration ability of GJA1 knockdown HBVSMC. Scale = 200 µm. The bar chart of I shows the proportion of migration HBVSMC (mean ± SD, n = 3 biologically independent samples). G Flow cytometric cell cycle analysis for the TUBA4B/TUBB4B overexpression HBVSMC after GJA1 knockdown. The bar chart of J shows the proportion of HBVSMC in G0/G1, S, and G2/M cell cycle phases (mean ± SD, n = 3 biologically independent samples).
