Fig. 4: Single-cell RNA sequencing indicates abnormalities in the abundance and function of VSMCs in MMD organoid.
From: Organoid Modeling and Single-Cell Profiling Reveal Smooth Muscle Cell Migration in Moyamoya Disease
A Representative immunofluorescence and three-dimensional (3D) reconstruction of HC and MMD vascular organoids. CD31, green; αSMA, red; DAPI, blue. Scale = 100μm. n = 3. B Schematic representation of single-cell RNA sequencing. A total of 85,392 single cells were captured, including organoids derived from 2 MMD patients and 2 HC. C A UMAP plot displaying all cells colored according to the 9 major cell types. D The proportion of different cell clusters within each organoid. E The percentage of each cell type in vascular organoids derived from MMD and HC. SingleR Single-cell RNA sequencing.
