Fig. 5: MMD vascular organoids exhibit an upregulation of TUBA4A and activation of the downstream GJA1/PI3K/AKT/KLF4 pathways.
From: Organoid Modeling and Single-Cell Profiling Reveal Smooth Muscle Cell Migration in Moyamoya Disease
A Immunofluorescence staining of smooth muscle cells during the vascular network phase in MMD organoids. B The weight and number of connections between smooth muscle cells and other cell types in the MMD organoids. C Immunofluorescence staining of TUBA4A in vascular organoids from MMD patients and HC. TUBA4A, red; DAPI, blue. n = 3. D Western blot showing the expression level of TUBA4A and TUBB4B in the vascular organoids. The bar chart shows the expression level of TUBA4A and TUBB4B (mean ± SD, n = 3 biologically independent samples). E GO enrichment analysis of smooth muscle cells in vascular organoids. GO Gene Ontology.
