Fig. 6: TUBA4A or TUBB4B overexpressed in HBVSMCs promotes the proliferation of HBECs through the KLF4 molecule.
From: Organoid Modeling and Single-Cell Profiling Reveal Smooth Muscle Cell Migration in Moyamoya Disease
A Ki67 staining of Human Brain Endothelial Cells (HBECs) after co-culture with treated HBVSMCs. The bar chart of B shows the proportion of proliferation HBEC (mean ± SD, n = 3 biologically independent samples). C Western blot showing the expression levels of TUBA4A, TUBB4B, and GJA1 in HBVSMCs under different treatment conditions in the SMC-EC co-culture system. The bar chart of D shows the expression level of GJA1 in HBVSMCs (mean ± SD, n = 3 biologically independent samples). E Western blot showing the expression levels of TUBA4A, TUBB4B, and GJA1 in HBECs under different treatment conditions in the SMC-EC co-culture system. The bar chart of F and G shows the expression level of GJA1 and KLF4 in HBECs, respectively (mean ± SD, n = 3 biologically independent samples). HBVSMC Human brain vascular smooth muscle cells, HBEC Human brain Endothelial cells, SMC-EC Smooth muscle cells-Endothelial cells.
