Fig. 5: Cross-sections through cryo-EM maps of phage 812 portal channel.
From: Genome anchoring, retention, and release by neck proteins of Staphylococcus phage 812
A, B Twelvefold-symmetrized reconstructions of the portal in the virion (A, EMD-18445) and genome release intermediate (B, EMD-18213) are shown as gray semi-transparent surface. Portal proteins are shown in light blue in cartoon representation. Blue arrows indicate the position of tunnel loops, and red asterisks denote DNA rings around the portal complex. C–E Asymmetric reconstruction of the portal channel of the phage 812 genome release intermediate (EMD-18372) viewed perpendicular to (C) and along (D, E) the tail axis. The portal complex is in light-blue cartoon representation, the adaptor complex in gold, and the DNA in red. The tunnel loops of portal proteins (residues 375–392) extend towards the DNA. The contacts with the DNA phosphate backbone are mediated by side chains of Lys381 (D) and the flexible loop Gly376–Gly379 (E), which are indicated by blue arrows.
