Fig. 7: Structure of terminus of phage DNA and tail tape measure protein in virion tail.
From: Genome anchoring, retention, and release by neck proteins of Staphylococcus phage 812
A An asymmetric reconstruction of the neck-to-tail junction (EMD-18516) is shown as gray semi-transparent surface. Adaptor proteins are shown in cartoon representation in gold, stopper proteins in magenta, tail terminator proteins in orange, stopper decoration proteins in forest green, terminator decoration proteins in steel blue, tail tube proteins in violet, and tail sheath proteins in light green. An ideal B-form dsDNA (in red) was placed into the helical density inside the tail tube channel. The first 14 residues of three TMPs (in turquoise) were fitted into the channel density starting at the fifth ring of the tail tube. B Comparison of reconstructed DNA densities in phage 812 tail channel of the virion and neck channel of the genome release intermediate with a B-form DNA model. The neck channel DNA has clear major (orange arrow) and minor (blue arrow) groove valleys typical for B-form DNA. The tail channel DNA has a deep major groove and a shallow minor groove caused by an off-center displacement of the base-pair densities, indicating a hybrid B/A form. C The reverse complement (RC) sequence (85 right-end base pairs) of the phage 812 long terminal repeat is aligned with the DNA density in panel A from the adaptor β-hairpin tube to the end of the genome.
