Fig. 4: Analyses of trajectories simulated in 30-dimensional PCA space reveal two classes of EMT trajectories. | Communications Biology

Fig. 4: Analyses of trajectories simulated in 30-dimensional PCA space reveal two classes of EMT trajectories.

From: Single cell snapshot analyses under proper representation reveal that epithelial-mesenchymal transition couples at G1 and G2/M

Fig. 4: Analyses of trajectories simulated in 30-dimensional PCA space reveal two classes of EMT trajectories.

a Trajectory simulation set-up for 12.5 pM TGF-β treated MCF10A cells with specified start (epithelial) and end (mesenchymal) region. Example trajectories from one round of 12.5 pM (b) and 25 pM (c) TGF-β simulations mapped onto the EMT-cell cycle coordinate representation. Mean trajectories from the d 12.5 pM and e 25 pM TGF-β simulations mapped on to the EMT-cell cycle coordinate representation. Mean trajectories for two clusters in the 3-d DC-EMT space for the 12.5 pM (f) and 25 pM (g) TGF-β simulations. Cell cycle phases were assigned using Revelio.

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