Fig. 5: Long-day exposure changes the expression levels of GluA1, GluA2 and Rab5c. | Communications Biology

Fig. 5: Long-day exposure changes the expression levels of GluA1, GluA2 and Rab5c.

From: Prolonged visual experience accelerates developmental synaptic downscaling via epigenetic regulation and Rab5c mediated AMPA receptor trafficking

Fig. 5: Long-day exposure changes the expression levels of GluA1, GluA2 and Rab5c.The alternative text for this image may have been generated using AI.

a Representative Western blots showing GluA1, GluA2, and Rab5c protein levels in tadpoles treated with 4LE, 12LE, and 20LE at 2, 5, and 8 dpl. b Quantitative analysis indicates a significant decrease in GluA1 and GluA2 expression in 20LE-treated tadpoles compared to 12LE at 5 and 8 dpl, whereas Rab5c is transiently upregulated at 2 dpl in 20LE. GluA1, GluA2 and Rab5c: n = 3–7 per group at each time point (two-way ANOVA with post hoc Fisher’s LSD test). c Representative Western blots of GluA1, GluA2, PSD95, HDAC2 and Rab5c in synaptosomal, cytosolic and total input fractions. d, e Synaptosomal analysis shows reduced GluA2 and increased Rab5c levels in 20LE tadpoles compared to 12LE. GluA1: n = 4, 4, 4; GluA2: n = 4, 4, 4; Rab5c: n = 5, 6, 5 for 4LE, 12LE, and 20LE. *p < 0.05; **p < 0.01 (one-way ANOVA with post hoc Dunnett’s test).

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