Fig. 8: Long-day exposure increases histone acetylation.

a Western blot analysis shows acetylation levels of H3K9Ac, H2BK5Ac and H4K8Ac in 4LE, 12LE, and 20LE groups at 2, 5, and 8 dpl. b–d Summary data reveal significantly elevated histone acetylation in 20LE brain compared to 12LE. 2 dpl/5 dpl/8 dpl: H3K9Ac: n = 9/6/7, 9/6/7, 9/6/7; H2BK5Ac: n = 8/4/4, 8/4/4, 8/4/4; H4K8Ac: n = 9/6/6, 9/6/6, 9/6/6 for 4LE, 12LE, and 20LE. *p < 0.05; **p < 0.01; ***p < 0.001 (two-way ANOVA with post hoc Fisher’s LSD test).