Fig. 9: Histone deacetylases regulate mEPSC amplitude, intrinsic excitability and AMPAR expression. | Communications Biology

Fig. 9: Histone deacetylases regulate mEPSC amplitude, intrinsic excitability and AMPAR expression.

From: Prolonged visual experience accelerates developmental synaptic downscaling via epigenetic regulation and Rab5c mediated AMPA receptor trafficking

Fig. 9: Histone deacetylases regulate mEPSC amplitude, intrinsic excitability and AMPAR expression.The alternative text for this image may have been generated using AI.

a Representative mEPSC traces from Ctrl-MO, HDAC2-MO, and HDAC3-MO neurons at 2 dpl. Scale bar: 20 pA, 20 s. b Quantification shows that HDAC2-MO and HDAC3-MO reduce mEPSC amplitudes. n = 6, 8, 11 for Ctrl-MO, HDAC2-MO, and HDAC3-MO. c Representative mEPSC traces from Ctrl-GFP, HDAC1-GFP, HDAC2-GFP and HDAC3-GFP neurons in 20LE tadpoles at 2 dpl. Scale bar: 20 pA, 20 s. d Summary data showing that Ctrl-GFP, HDAC1-GFP, HDAC2-GFP, and HDAC3-GFP increase mEPSC amplitudes. n = 23, 17, 14, 16 for Ctrl-GFP, HDAC1-GFP, HDAC2-GFP, and HDAC3-GFP. e Representative traces of current injection-induced spikes in control and TSA-treated neurons. n = 10, 9 for Ctrl and TSA. Scale bar: 40 mV, 40 ms. f Summary data showing that TSA treatment increases intrinsic excitability compared to control neurons. g–i Western blot analysis showing that TSA treatment downregulates GluA1 and GluA2 protein expression. GluA1: n = 7, 7; GluA2: n = 4, 4 for Ctrl and TSA. j–m Western blot analysis showing that HDAC2-MO decreases GluA2 expression, whereas HDAC3-MO downregulates GluA1 and GluA2 but increases Rab5c expression. Rab5c and GluA2: n = 4 per group; GluA1: n = 3 per group. n–q Western blot analysis showing that Rab5c-MO increases H3K9Ac, H2BK5Ac, and H4K8Ac levels. H3K9Ac: n = 8 per group; H2BK5Ac: n = 3 per group; H4K8Ac: n = 4 per group. *p < 0.05; **p < 0.01; ***p < 0.001.

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