Fig. 2: piR48444 knockdown promotes osteogenic differentiation across multiple mesenchymal stem cell types. | Communications Biology

Fig. 2: piR48444 knockdown promotes osteogenic differentiation across multiple mesenchymal stem cell types.

From: PiR48444 inhibits MSC osteogenic differentiation and bone regeneration via targeting METTL7A/eIF4E-mediated BMP2 m6A methylation

Fig. 2: piR48444 knockdown promotes osteogenic differentiation across multiple mesenchymal stem cell types.

A–D SHEDs were transfected with a piR48444 knockdown (piR48444-KD) or overexpression (piR48444-OE) construct. A, B Representative images (left) and quantification (right) of ALP staining at day 7 and Alizarin Red S (ARS) staining at day 14. C, D mRNA expression levels of the osteogenic markers ALP, RUNX2, OCN, COL1A1, and BSP at day 7, measured by RT-qPCR. E–P The effects of piR48444 modulation were validated in BMSCs (E–H), PDLSCs (I–L), and ASCs (M–P). E, I, M Representative ALP staining at day 7 following piR48444-KD. F, J, N Osteogenic gene expression at day 7 following piR48444-KD. G, K, O Representative images and quantification of ALP staining at day 7 following piR48444-OE. H, L, P Osteogenic gene expression at day 7 following piR48444-OE. All quantitative data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined by a two-tailed Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001). KD knockdown, OE overexpression.

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