Fig. 8: METTL7A regulates BMP2 expression through interacting with eIF4E. | Communications Biology

Fig. 8: METTL7A regulates BMP2 expression through interacting with eIF4E.

From: PiR48444 inhibits MSC osteogenic differentiation and bone regeneration via targeting METTL7A/eIF4E-mediated BMP2 m6A methylation

Fig. 8: METTL7A regulates BMP2 expression through interacting with eIF4E.

A, B Bioinformatic and RT-qPCR analysis of eIF4EBP1 and eIF4E in SHED during osteogenic differentiation. C The expression of eIF4EBP1 and eIF4E in piR48444-KD SHED. D The binding prediction analysis of METTL7A and eIF4E by ClusPro and PyMOL. E Co-IP analysis of the binding between METTL7A and eIF4E. F Expression pattern of eIF4E in eIF4E-OE SHED. G The expression levels of osteogenic genes, including ALP, RUNX2, and OCN, at day 7. H The staining of ALP and quantification at day 7. I Expression pattern of eIF4E in eIF4E-KD SHED at day 7. J The expression levels of osteogenic genes, including ALP, RUNX2, and OCN, at day 7. K The staining of ALP and quantification at day 7. L, M BMP2 expression and quantification in eIF4E-OE and eIF4E-KD SHED. N The expression levels of osteogenic genes, including ALP, RUNX2, and OCN, at day 7. O The staining of ALP and quantification at day 7. Data are presented as mean ± SD (n = 3). Statistical significance was determined by Student’s t test (A–C, F, I, M), and one-way ANOVA (G, H, J, K, N, O). Significance levels: *p < 0.05, **p < 0.01, and ***p < 0.001. OE overexpression, KD knockdown.

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