Fig. 6: miR-122-5p induces ferroptosis of Leydig cells by targeting Scd2. | Communications Biology

Fig. 6: miR-122-5p induces ferroptosis of Leydig cells by targeting Scd2.

From: Obesity impairs spermatogenesis via Leydig cell ferroptosis induced by liver-derived exosomal miR-122-5p

Fig. 6: miR-122-5p induces ferroptosis of Leydig cells by targeting Scd2.The alternative text for this image may have been generated using AI.

A Relative luciferase activity in different treatment groups (n = 3). B Relative miR-122-5p expression in Leydig cells with different treatments (n = 3). C Relative Scd2 mRNA expression in Leydig cells (n = 3). D Leydig cell viability of si-NC- or si-SCD2-treated groups (n = 6). E Relative ferrous ion levels of Leydig cells. F-a MMP of Leydig cells (n = 3), F-b Fluorescence intensity of the sample (Scale bar = 100 µm). G Relative MDA levels of Leydig cells (n = 3). H Relative testosterone concentration in Leydig cell supernatants (n = 3). I-a Relative ROS levels of Leydig cells treated with si-NC or si-SCD2 (n = 3), I-b Fluorescence intensity of the sample (Scale bar = 100 µm). J Cell viability of Leydig cells transfected with mimics-NC, mimics-miR-122-5p, OE-NC, or OE-SCD2 (n = 6). K Relative ferrous ion levels of Leydig cells transfected with mimics-NC, mimics-miR-122-5p, OE-NC, or OE-SCD2 (n = 3). L Relative MDA levels of Leydig cells transfected with mimics-NC, mimics-miR-122-5p, OE-NC, or OE-SCD2 (n = 3). M-a MMP of Leydig cells transfected with mimics-NC, mimics-miR-122-5p, OE-NC, or OE-SCD2 (n = 3), M-b Fluorescence intensity of the sample (Scale bar = 100 µm). N Relative testosterone concentration in Leydig cell supernatants from groups treated with mimics-NC, mimics-miR-122-5p, OE-NC, or OE-SCD2 (n = 3). O-a Relative ROS levels of Leydig cells transfected with mimics-NC, mimics-miR-122-5p, OE-NC, or OE-SCD (n = 3), O-b Fluorescence intensity of the sample (Scale bar = 100 µm). Data information: Results are expressed as mean values ± SD. Data were analyzed using an unpaired Student’s t-test or one-way ANOVA. *p < 0.05, **p < 0.01. Significance between groups was indicated by labeling with different letters; data labeled with the same letter indicate no significant difference.

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