Fig. 2: Microscopic observation of gut architecture in mouse tissue.
From: enGLOW 3D microscopy of the enteric nervous system in cleared human and mouse gut
a Representative photographs of 2-cm-long segments of uncleared (left) and cleared (right) mouse distal colon over a millimeter paper. b Hematoxylin and eosin (H&E) stained cryosection of mouse colon imaged in brightfield with 40X objective in a slide scanner. c Circumferential reslice of two-channel image of mouse colon acquired in mesoSPIM with 2X magnification. Tissue architecture is visible by autofluorescence (cyan) and enteric neuron cell bodies are labeled with HuCD (magenta). In (b) and (c), the identifiable layers are annotated. d 3D rendering of mouse colon in autofluorescence channel acquired with mesoSPIM 2X magnification. e Longitudinal slice (i), with close-up of identifiable layers (ii) and 3-μm-thick circumferential reslice (iii) of mouse colon. Arrow in (ii) points to detachment of submucosa from muscle that can occur during sample preparation. f Close-up of notable structures of the gastrointestinal tract, including the villi and papilla de Vater (arrow) of the small intestine (i), autofluorescence of neurons (arrows, ii) and perfused blood vessels (arrow, iii). Abbreviations: LM longitudinal muscle, CM circular muscle, SM submucosa, MM muscularis mucosae, LP lamina propria, EP epithelium.
