Fig. 4: Four key cellular components of the ENS in 3D volumes and virtually dissected layers.
From: enGLOW 3D microscopy of the enteric nervous system in cleared human and mouse gut
a HuCD labeling of neuron cell bodies (magenta) in distal colon imaged with mesoSPIM 2X magnification. a-i Longitudinal slice view of close-up COLM acquisition of HuCD. a-ii Maximum intensity projection of gut wall COLM acquisition of HuCD. b Virtually flattened HuCD labeling in longitudinal muscle, myenteric plexus, circular muscle and submucosal plexus layers of tissue. c Tuj1 labeling of neuronal fibers (orange) in distal colon imaged with mesoSPIM 2X magnification. c-i Longitudinal slice view of close-up COLM acquisition of Tuj1. c-ii Maximum intensity projection of gut wall COLM acquisition of Tuj1. d Virtually flattened Tuj1 labeling. e S100β labeling of glia (yellow) in distal colon imaged with mesoSPIM 2X magnification. e-i Longitudinal slice view of close-up COLM acquisition of S100β. e-ii Maximum intensity projection of gut wall COLM acquisition of S100β. f Virtually flattened S100β labeling. g C-kit labeling of interstitial cells of Cajal (green) in distal colon imaged with mesoSPIM 2X magnification. g-i Longitudinal slice view of close-up COLM acquisition of c-kit. g-ii Maximum intensity projection of gut wall COLM acquisition of c-kit. h Virtually flattened c-kit labeling. Cyan channel is tissue autofluorescence in (a), (c), (e) and (g). i Percentage of area in field of view occupied by specific signal for each labeled structure. If not specified, scale bars are 100 μm. n = 3 regions of interest in n = 1 animal. Quantitative data used for plotting can be found in tables within Supplementary Data 1. Statistics: Error bars are standard error of the mean.
