Fig. 5: Enteric nervous system at the network scale in healthy human colon resections.
From: enGLOW 3D microscopy of the enteric nervous system in cleared human and mouse gut
a Representative photographs of colon surgical resections before (left) and after (right) enGLOW processing. b 2D cross-section of autofluorescence and neurons labeled with HuCD in 3.2-mm-thick colon acquired with mesoSPIM 2X magnification, where layers from the serosa to the mucosa are visible. c 3D rendering of 1.28 cm by 1.33 cm area of healthy colon resection of the same sample as in (b) with orthogonal view in dashed box. d Top: 2D flattened projections of high-resolution images acquired with COLM 4X magnification of HuCD-labeled neurons to separate cells in the submucosal and myenteric plexuses. Bottom: Depth mapping of the layers from serosa to submucosa (first 180 image planes equal to 585 μm). e 2D cross-section of neuron cytoskeleton labeled with Tuj1 in 3.3-mm-thick colon acquired with mesoSPIM 2X magnification where layers from the muscle to the mucosa are visible. f 3D rendering of 1.28 cm by 1.33 cm area of healthy colon resection with Tuj1 labeling of neuron fibers with orthogonal view in dashed box. g-i High-resolution 2D maximum intensity projections acquired with COLM 4X magnification. g Tuj1 signal wrapping around blood vessels. h Tuj1 signal in the epithelium enabling identification of specialized neuropod cells. i Dense network of innervation throughout the mucosa.
