Fig. 1: Mis-expression of TOC1 leads to loss of temporal variation in immunity to Botrytis cinerea under both constant light and diurnal conditions. | Communications Biology

Fig. 1: Mis-expression of TOC1 leads to loss of temporal variation in immunity to Botrytis cinerea under both constant light and diurnal conditions.

From: The core clock transcription factor TOC1 binds directly to defence gene promoters regulating immunity in Arabidopsis

Fig. 1: Mis-expression of TOC1 leads to loss of temporal variation in immunity to Botrytis cinerea under both constant light and diurnal conditions.The alternative text for this image may have been generated using AI.

Detached leaves from four-week-old plants grown under a 16 h light and 8 h dark cycle were inoculated with B. cinerea spores (A) under constant light conditions at subjective dawn (CT24) or subjective night (CT42) and (B) under diurnal conditions at dawn (ZT0) or night (ZT18) and lesion size measured at 72 hpi. Data shown are mean values ± SEM, with the number of leaves (from independent plants) inoculated indicated within the bars. Under LL (A) time of inoculation (F = 11.6, p = 0.0009, η2 = 0.02), plant genotype (F = 180.8, p < 0.0001, η2 = 0.78) and the interaction between these factors (F = 4.1, p 0.008, η2 = 0.02) had a significant effect on lesion size in a two-way ANOVA. Under LD (B) time of inoculation (F = 24.6, p < 0.0001, η2 = 0.05), plant genotype (F = 87.3, p < 0.0001, η2 = 0.54) and the interaction between these factors (F = 3.3, p 0.021, η2 = 0.02) had a significant effect on lesion size in a two-way ANOVA. Mean lesion sizes with different letters are significantly different (p < 0.05) as determined by Fisher LSD post-hoc test. Results shown are from one experiment representative of two (A) or three (B) independent experiments.

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