Fig. 3: Plants mis-expressing TOC1 show altered expression of ORA47 and WRKY33. | Communications Biology

Fig. 3: Plants mis-expressing TOC1 show altered expression of ORA47 and WRKY33.

From: The core clock transcription factor TOC1 binds directly to defence gene promoters regulating immunity in Arabidopsis

Fig. 3: Plants mis-expressing TOC1 show altered expression of ORA47 and WRKY33.The alternative text for this image may have been generated using AI.

Detached leaves from four-week-old plants grown under a 16 h light and 8 h dark cycle were inoculated at dawn with Botrytis cinerea spore suspension or mock control. Leaf tissue was harvested at 18 hpi and relative expression of ORA47 and WRKY33 determined by qPCR with normalisation to the geometric mean of ACTIN2 and MON1 expression. Pathogen challenge (F = 101.6, p < 0.0001, η2 = 0.56) and plant genotype (F = 19.3, p < 0.0001, η2 = 0.32) had a significant effect on ORA47 expression in a two-way ANOVA. Pathogen challenge (F = 68.5, p < 0.0001, η2 = 0.47) and plant genotype (F = 18.7, p < 0.0001, η2 = 0.39) had a significant effect on WRKY33 expression in a two-way ANOVA. Mean expression values with different letters are significantly different (p < 0.05) as determined by Fisher LSD post-hoc test. The data presented are mean values ± SEM from three biological replicates. This experiment was performed once.

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