Fig. 4: TOC1 occupancy of ERF4, ORA47, ORA59 and WRKY33 promoters is modulated by both pathogen challenge and the presence of MYC2.

Two-week old TMG and TMG myc2 seedlings were inoculated at ZT0 with B. cinerea spore suspension or mock control. A–D Seedlings were cross-linked at ZT18 and chromatin immunoprecipitated with an anti-GFP antibody. ChIP-qPCR analysis of TOC1 occupancy of a G-box containing region of the promoter and an exonic region of each TOC1-target gene was performed (position relative to transcription start site is indicated). Values shown are mean percentage input ± SEM from three independent experiments (two for ERF4 and ORA47 exonic regions). Mean values with different letters are significantly different (p < 0.05) as determined by Fisher LSD post-hoc test. No antibody control values and results of two-way ANOVA for each target gene are presented in Supplementary Tables 4 and 5, respectively. E–H Seedlings were harvested at ZT18 and relative expression of each target gene was determined by qPCR with normalization to the geometric mean of ACTIN2 and MON1 expression. The data presented are mean expression values ± SEM from three biological replicates. Mean values with different letters are significantly different (p < 0.05) as determined by Fisher LSD post-hoc test. Results of two-way ANOVA for each target gene are presented in Supplementary Table 5.