Table 2 Primers used for ChIP experiments

From: The core clock transcription factor TOC1 binds directly to defence gene promoters regulating immunity in Arabidopsis

Gene

Region

Position

Primer sequences

Conc (nM)

Amplicon (bp)

ERF4

G-box

-183

AACGCTTGGTGAGGTTAAA

400

139

   

CTCTTACAAAACACCAGCGG

  
 

Exon

615

GACTCTGATTCGTCATCGGTCG

400

103

   

AGGCCTGTTCCGATGGAGG

  

ORA47

G-box

-143 -69

CAAACGTGCCTAAACAAATAGAG

300

204

   

GAAGTGGCAAGTGAGGAAGAA

  
 

Exon

603

TTTGATTCCTTCTCCGACGACTTC

500

100

   

CATCTAATAAACTCTCTCCTCCGTAATC

  

ORA59

G-box

-194

CATCTATTTTGTCGGGAATAC

300

93

   

ATGAGATACACAGAAGTGGGTGA

  
 

Exon

701

ACATCTTCTCCTTCTTCCTCC

400

98

   

TCTTGCGTCATAACAACACTCTG

  

WRKY33

G-box

-1120

CGGATCAACAAACACTGCATAG

500

105

   

GCACGATATGTGGTATGGTTCT

  
 

Exon

1407

AGTCTGATTCCTTTGGGATGC

500

93

   

TGCTGACAATCGATGAGCCT

  

CCA1

G-box

-193

TGTAGTGAACCGCACGAGAA

500

122

   

CGTTAATCTCTCCCACCAGTTT

  
  1. All G-boxes are the canonical CACGTG sequence. Position relative to transcriptional start site is given to the 5’ end of the canonical G-box sequence and to 5’ end of the exonic amplicon. The annealing temperature used for all primer sets was 60 °C, except for ORA59 which was run at 55 °C.
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