Fig. 1: Design and development of the FcRn-immobilized column. | Communications Biology

Fig. 1: Design and development of the FcRn-immobilized column.

From: Integrative structural insights into the IgG-FcRn interactions revealed by engineered FcRn-immobilized affinity chromatography

Fig. 1: Design and development of the FcRn-immobilized column.The alternative text for this image may have been generated using AI.

a The mutation sites of FcRn are shown. The six mutation sites in FcRn are colored in yellow. K272E is not displayed in the figure because the structure of the FcRn C-terminus was not determined in the crystal structure. b Thermal stability of FcRn wild type and mutant. DSC thermogram of wild type (black) and mutant (blue) FcRn are shown. c A schematic view of IgG, FcRn, and resin is represented. The model was assembled using the crystal structures of human FcRn (steel blue), β2m (medium aquamarine), Fc (light gray), Fab H chain (light salmon), and Fab L chain (lemon chiffon). PDB IDs of each structure are as follows: adalimumab Fab, 4NYL; Fc, 4w4N; and FcRn, 4N0U. The image of the resin is shown as a sphere colored in teal.

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