Fig. 2
From: Synthesis of disulfide-rich heterodimeric peptides through an auxiliary N, N-crosslink
Synthetic route to insulin-like peptides. i Synthesis of folded single-chain insulin-like peptides enabled by an intermediary N–N chemical ligation followed by intramolecular disulfide bond formation. ii Synthesis of two-chain insulin-like peptides by DKP-mediated linker cleavage of the folded single-chain insulin-like peptides. iii A one-pot relaxin synthesis through sequential ligation, folding, DKP cleavage, and pGlu formation with a single purification
