Fig. 2
From: Hierarchical self-assembly and emergent function of densely glycosylated peptide nanofibers
GQ11 nanofibers laterally associate and align in crowded conditions. GQ11 nanofibers were dispersed when viewed using transmission electron microscopy in a water and b phosphate-buffered saline (PBS). GQ11 nanofibers were aligned in the presence of Ficoll when viewed using c, d transmission electron microscopy and e cryo-electron microscopy. f GQ11 nanofibers selectively bound wheat germ agglutinin (WGA), a GlcNAc-binding lectin, but not Concanavalin A (ConA) or bovine serum albumin (BSA), suggesting carbohydrates were on the nanofiber surface and accessible to the surrounding aqueous environment. Data presented as mean ± standard deviation (n = 3). Inset: Co-localization of GQ11 nanofibers and WGA-coated gold beads (scale bar = 50 nm). GQ11 nanofibers were also aligned in the presence of g BSA, h, i ConA, and j trifluoroethanol (TFE) when viewed using TEM (scale bar = 100 nm)
