Fig. 3

Growth of AOT or SDBS/DA (1:1) GUVs in response to the micro-injection of AOT or SDBS micelles under various conditions. a Experiments with AOT GUVs for the different conditions listed in Table 1. Red diamonds (entry #1): Micro-injection of 2.0 M H₂O₂ and 20 mM AOT micelles dissolved in water to 3.0 mM AOT GUVs prepared in 20 mM NaH₂PO₄ pH = 4.3 solution with 4.0 mM aniline and 0.92 μM HRP. Dark blue closed circles (entry #2): AOT GUV suspension without aniline and without HRP. All other conditions are kept the same as entry #1. Light blue triangles (entry #3): AOT GUV suspension without aniline. All other conditions are kept the same as entry #1. Violet squares (entry #4): AOT GUV suspension without HRP. All other conditions are kept the same as entry #1. Orange open circles (entry #5): The micro-injection solution did not contain H₂O₂. All other conditions are kept the same as entry #1. b Experiments demonstrating the selectivity of the GUV growth, see also Table 2. Blue circles (i): Micro-injection of 2.0 M H₂O₂ and 20 mM AOT SUVs prepared in 20 mM NaH₂PO₄ solution (pH = 4.3) to 3.0 mM AOT GUVs prepared in 20 mM NaH₂PO₄ pH = 4.3 solution with 4.0 mM aniline and 0.92 μM HRP (vesicle growth observed). Green triangles (ii): Micro-injection of 2.0 M H₂O₂ and 100 mM SDBS micelles prepared in 20 mM NaH₂PO₄ solution to AOT GUVs. All other conditions are kept the same as for (i) (vesicle growth observed with delay). Orange squares (iii): Micro-injection of 2.0 M H₂O₂ and 20 mM AOT SUVs prepared in 20 mM NaH₂PO₄ solution (pH = 4.3) to 3.0 mM SDBS/DA (1:1) GUVs prepared in 20 mM NaH₂PO₄ pH = 4.3 solution with 4.0 mM aniline and 0.92 μM HRP (slow vesicle growth observed). Red diamonds (iv): Micro-injection of 2.0 M H₂O₂ and 100 mM SDBS prepared in 20 mM NaH₂PO₄ solution to SDBS/DA (1:1) GUVs. All other conditions are kept the same as for (iii) (vesicle growth observed). The error bars indicate standard deviations estimated from three different experiments