Fig. 5
From: Photocatalytic proximity labelling of MCL-1 by a BH3 ligand
Selectivity of Ru(II)(bpy)3-NOXA-B 1 for MCL-1 over BCL-xL. a Fluorescence anisotropy competition experiment for the inhibition of the MCL-1/FITC-NOXA-B interaction (red) and BCL-xL/FITC-BID interaction (black) by peptide 1. Error bars represent the standard deviation of three repeats. b SDS-PAGE gel showing selective labelling of MCL-1 in a 1:1 mixture of MCL-1 and BCL-xL (lane 1). BCL-xL is not labelled in a solution of the protein on its own (lane 5). Conditions: 5 µM protein, 1 µM RTA 2, 10 µM APS, 1 min irradiation, 50 mM (NH4)HCO3 (pH 7.4). Pictures of complete gels are shown in the Supplementary Fig. 33.
