Fig. 7: Observation of 3n in A549 cells. | Communications Chemistry

Fig. 7: Observation of 3n in A549 cells.

From: Development of a 1,3a,6a-triazapentalene derivative as a compact and thiol-specific fluorescent labeling reagent

Fig. 7

Living cells were cultured with 10 µM or 25 µM of 3n (a, b), cultured with 10 µM or 25 µM of 2a containing 0.1% DMSO as a control (c, d), left untreated (e), or cultured with 25 μM of 7n containing 0.1% DMSO (f). The fluorescence of 3n inside the cells was clearly visualized (a, b), whereas that inside the cells treated by TAP-VK1 (c, d), the untreated cells (e) and the cells treated with R8 peptide (f) was not visualized under several concentrations. Cell nuclei were stained by PI (propidium iodide; red fluorescence) for detailed fluorescence observation, and 3n was observed in the cytoplasm. The uptake of 3n was monitored by confocal microscopy (ZEISS LSM700; Carl Zeiss) in a fluorescence image with excitation and emission wavelengths of 405 and 490 nm, respectively (a–f). Scale bar = 20 µm.

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