Fig. 6: Bead-bait hybridization assay for detection of KRAS G12D mutation.
From: Optical and theoretical study of strand recognition by nucleic acid probes
a An approach to developing genotyping probes for KRAS G12D, b Main steps of the assay including (I) formation of bead-capturing probe followed by catching or “purification” of the target RNA by washing the non-binding genetic material; (II) hybridization with specific linker probe to link target RNA with ct DNA followed by washing step; (III) ct DNA addition and linkage to the linker probe followed by a washing step then DMSO denaturation and separation from the bead-capturing probe (IV) re-establishment of the hybridization conditions with buffer exchange and fluorescence readout using intercalating Eva Green dye. LNA (L) locked nucleic acid.
