Fig. 4: Characterization of 3D T158HMjFer superlattices. | Communications Chemistry

Fig. 4: Characterization of 3D T158HMjFer superlattices.

From: Converting histidine-induced 3D protein arrays in crystals into their 3D analogues in solution by metal coordination cross-linking

Fig. 4

a TEM images of 3D assembly of T158HMjFer formed by a combination of π–π stacking interactions and Ni2+ coordination. b, c Enlargements of protein superlattices. d SAXS analyses of 3D T158HMjFer superlattices. The (hkl) values in radially averaged 1D SAXS data are labeled above the peaks. The experimental curve (black) matches the simulated pattern (blue dash) well, revealing a simple cubic (sc) structure. The inserted image in d is the 2D SAXS pattern of T158HMjFer assemblies. e Miller indices of assigned reflections for the sc structure versus measured q-vector positions for indexed peaks yield unit cell dimensions of a = 11.7 nm. The inserted image in e is a unit cell of the superlattice composition. TEM conditions: 1.0 µM of T158HMjFer protein in 25 mM Tris-HCl, pH 8.0 containing 500 mM NaCl, 0.7 mM of Ni2+. SAXS samples were prepared by centrifugation of 3D assemblies.

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