Fig. 2: Time-resolved absorption and Raman spectra of miRFP670/C20A at pH 8.0.
From: Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome
a Selected time-resolved absorption spectra at the times indicated. b stimulated Raman spectrum of biliverdin (BV) prior to addition of apoprotein (gray line); Evolution-associated spectra (EAS) of globally fitted time-resolved absorption spectra. The indicated time constants denote the lifetime by which each EAS evolves into the next one. c Stimulated Raman spectrum of BV in a buffer solution with an 800 nm Raman pump. d–f EAS of globally fitted time-resolved stimulated Raman spectra with an 800 nm Raman pump. The gray lines denote the stimulated Raman spectrum BV in buffer solution reproduced from (c).
