Fig. 3: Accumulation of uranine in trapped liposomes.

a–l Three types of (a, e, i) time courses of GFIlipo (blue) and GFIBG (red) upon the exposure to low pH solution and following washout with (b–d, f–h, j–l) representative EFM images. The contrast of EFM images was modified for the visual readability. Scale bars: 10 µm. m Time course of the ratios of GFIlipo to GFIBG exposed to a 0.5 µM uranine/1 mM fructose solution. n Diagram showing the result of the numerical simulation on the time course of GFIlipo (red line) plotted with the experimental results (colored dots, liposomes; black line, background). o Time course of the ratio of GFIlipo to GFIBG at a cycle of sequential exposure (5 µM uranine/1 mM fructose; denoted as gray box) and following washout.