Fig. 2: Secondary and tertiary structure of GFP in aqueous ionic liquid solutions. | Communications Chemistry

Fig. 2: Secondary and tertiary structure of GFP in aqueous ionic liquid solutions.

From: Revealing the complexity of ionic liquid–protein interactions through a multi-technique investigation

Fig. 2

a Far-UV CD spectra (mean residue ellipticity (mre) against wavelength) showing retention of β-sheet structure. b UV/Vis spectroscopy absorbance data (absorbance against wavelength) showing the GFP chromophore is in its native state. c Fluorescence intensity (a.u.) at 525 nm (error bars correspond to standard deviations, n = 3 — full data shown in Supplementary Fig. 1, and Supplementary Table 1), and (d) small-angle X-ray scattering (SAXS) profiles (separated for clarity) fitted with a cylinder model for GFP dissolved in water (black), 1 M [bmpyrr][OAc] (green), 1 M [bmpyrr]Cl (blue), and 1 M [bmpyrr][OTf] (purple) at 25 °C.

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