Fig. 2: Biochemical and biophysical analyses of wild-type and fluorinated KDM5A PHD3 fingers. | Communications Chemistry

Fig. 2: Biochemical and biophysical analyses of wild-type and fluorinated KDM5A PHD3 fingers.

From: Mechanism of biomolecular recognition of trimethyllysine by the fluorinated aromatic cage of KDM5A PHD3 finger

Fig. 2

a 15% Tris-Tricine SDS-PAGE showing the purified, untagged KDM5A PHD3 fingers. From left to right: Wild-type KDM5A, 5F-KDM5A, 6F-KDM5A and 5,6diF-KDM5A. b ESI-MS data showing the masses of the purified KDM5A PHD3 fingers. The wild-type protein has a mass of 7170.1 Da, whereas the 5F and 6F alloproteins show a mass increase of +35.9 and +35.8 Da, respectively, and 5,6diF shows an increase of +71.9 Da. c CD spectra showing the purified PHD3 fingers over a range of 190–260 nm. Black: Wild-type KDM5A, red: 5F-KDM5A, green: 6F-KDM5A and blue: 5,6diF-KDM5A. d Melting temperatures of KDM5A PHD3 fingers obtained by DSF.

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